《植物生理学报》 2022, 58(4): 631-642

植物UDP-糖基转移酶(UGT)的功能及应用前景

于安东¹，刘琳²，龙瑞才¹，康俊梅¹，陈林¹，杨青川¹，李明娜^1,*

¹中国农业科学院北京畜牧兽医研究所, 北京100193；²巴彦淖尔市农牧业科学研究所, 内蒙古巴彦淖尔015000

通信作者：李明娜；E-mail: limingna@caas.cn

摘　要：

糖基化是植物的普遍修饰反应, 经修饰获得的糖基化产物具有不同的生物学功能, 使得糖基化反应成为影响及调节植物生长代谢平衡的重要机制。植物尿苷二磷酸糖基转移酶(uridine diphosphate glycosyltransferase, UGT)属于糖基转移酶一号家族(GT family 1), 主要以植物中最常见的尿苷二磷酸(UDP)-糖类为活化糖基分子供体, 以类黄酮、酚酸、萜类以及植物激素等小分子化合物为糖基受体。由于UGT催化的底物种类繁多, 产物活性差异较大, 其催化的糖基化反应在植物生长发育及代谢调节过程中具有十分重要的作用。本文概述了植物UGT家族的蛋白结构、特征及催化底物的相关信息, 由UGT参与植物的次生代谢、激素调控、逆境响应与脱毒反应四个方面重点总结了UGT的功能作用, 同时对UGT在调控作物农艺性状中的作用和应用前景进行了探讨和展望, 旨在为植物UGT的功能研究及生产应用提供一定的参考。

关键词：UDP-糖基转移酶(UGT); 糖基化修饰; 次生代谢物; 激素调控; 抗逆性

收稿：2021-11-01   修定：2022-01-11

资助：中央级公益性科研院所基本科研业务费专项(2020-YWF-YB-05)、中国农业科学院科技创新工程(ASTIP-IAS14)和“科技兴蒙”行 动重点专项(NMKJXM202110-5)

Function and application prospect of plant UDP-glycosyltransferase (UGT)

YU Andong¹, LIU Lin², LONG Ruicai¹, KANG Junmei¹, CHEN Lin¹, YANG Qingchuan¹, LI Mingna^1,*

¹Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; ²2Bayannur Institute of Agriculture and Animal Husbandry Sciences, Bayannur, Inner Mongolia 015000, China

Corresponding author: LI Mingna; E-mail: limingna@caas.cn

Abstract:

Glycosylation is a ubiquitous modification reaction of plants. The modified glycosylated products have various biological functions, making glycosylation become an important mechanism affecting and regulating the balance of plant growth and metabolism. Plant uridine diphosphate glycosyltransferase (UGT), belonging to GT faminy 1, mainly takes the most common uridine diphosphate (UDP)-sugars in plants as activated glycosyl molecular donors, and small molecular compounds such as flavonoids, phenolic acids, terpenes and plant hormones as glycosyl receptors. The glycosylation reactions catalyzed by UGT play an essential role in plant growth and development and metabolic regulation due to the wide variety of substrates and the differenatial of product activities. In this paper, the protein structure, and characteristics and catalytic substrate information of plant UGT family are summarized; the functional roles of  UGT are concluded by four aspects of secondary metabolism, hormone regulation, stress response and detoxification reaction; the prospects of the role and application of UGT in regulating agronomic traits of crops are also discussed and expected. Our goal is to provide pivotal references and theoretical basis for the functional study and production in application of plant UGT family.

Key words: UDP-glycosyltransferase (UGT); glycosylation modification; secondary metabolite; hormone regulation; stress resistanc